A0438

Sigma

 

Alcohol Oxidase from Hansenula sp.

vacuum-dried powder, ≥1 units/mg protein (Bradford)

Synonym:Alcohol:oxygen oxidoreductase
CAS Number:9073-63-6
Enzyme Commission (EC) Number:1.1.3.13   ( BRENDA | IUBMB )
EC Number:232-971-3
MDL number:MFCD00130452

References

Reviews

Description

Unit DefinitionOne unit will oxidize 1.0 μmole of methanol to formaldehyde per min at pH 7.5 at 25 °C.
Biochem/physiol ActionsCatalyzes the oxidization of primary alcohols to aldehydes.

Molecular Weight: ~600 kDa
Optimum pH: 8.5
pH Range: 5.5-8.5

Km values with the following substrates:
Allyl alcohol 33.3 mM
Benzyl alcohol 26.6 mM
Ethanol 16.2 mM
Formaldehyde 2.6 mM
Methanol 2.15 mM
N-Butanol 166 mM
n-Propanol 66 mM

Cofactor: FAD

Inhibitors: 1,4-butynediol (irreversible), propargyl alcohol (irreversible), cyclopropanol, cyclopropanone (suicide substrate), formaldehyde, H2O2, hydroxylamine, KBr, KCN, methanol (substrate inhibitor), NaN3, PCMB, propynal, urea, 4-chloromercuribenzoic acid

Properties

formvacuum-dried powder
storage temp.−20°C

Safety

Personal Protective EquipmentEyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany3

Related Products

InhibitorC5913, 4-Chloromercuribenzoic acid